Complementary DNA Hybridization Analysis of Double‐stranded RNAs Associated with Cucumber Mosaic Virus‐As Strain

Abstract

AbstractVirus‐specific double‐stranded (ds) RNAs were isolated from leaves of Nicotiana tabacum cv. Xanthi‐nc infected with the As or Y strains of cucumber mosaic virus (CMV). These dsRNAs on agarose gels produced 5 bands corresponding, respectively to three genomic RNAs (dsRNAI, −2, −3), the subgenomic mRNA for coat protein (dsRNA4). and satellite dsRNA (dsRNA5) with estimated molecular weights of respectively, 2.5, 2.4, 1.45, 0.69 and 0.28 × 103 kD. In northern‐blot analysis, a 1 kbp complementary DNA (cDNA) to CMV‐As RNA4 hybridized strongly with dsRNA3 and dsRNA4 extracted from CMV‐As infected tobacco, but did not hybridize with dsRNAI, dsRNA2, or satellite dsRNA (dsRNA5). In slot‐blot analysis, the cDNA hybridized strongly with virus‐,specific dsRNAs isolated from CMV infected plants, but not with dsRNAs of TMV‐Korean common, potato virus Y‐VN, or total RNA of healthy tobacco plants.