Abstract
In this study, the effect of sustainable probiotics on Campylobacter jejuni colonization and gut microbiome composition was evaluated using chicken as a model organism. Chickens were given Lactobacillus casei over-expressing myosin-cross-reactive antigen (LC+mcra). LC+mcra can generate bioactive compounds in larger quantity including conjugated linoleic acid. A total of 120 chickens were used in duplicate trials to investigate the effectiveness of LC+mcra in decreasing C. jejuni colonization by means of kanamycin resistant strain compared to the control group. We observed that LC+mcra can efficiently colonize various parts of the chicken gut and competitively reduce colonization of natural and challenged C. jejuni and natural Salmonella enterica. LC+mcra was found to reduce C. jejuni colonization in cecum, ileum and jejunum, by more than one log CFU/g when compared to the no-probiotic control group. Furthermore, 16S rRNA compositional analysis revealed lower abundance of Proteobacteria, higher abundance of Firmicutes, along with enriched bacterial genus diversity in gut of LC+mcra fed chicken. Decreased contamination of drinking water by C. jejuni and S. enterica was also observed, suggesting a potential function of reducing horizontal transfer of enteric bacteria in poultry. Outcomes of this study reveal high potential of LC+mcra as sustainable approach to decrease colonization of C. jejuni and S. enterica in poultry gut along with other beneficial attributes.
Highlights
In this study, the effect of sustainable probiotics on Campylobacter jejuni colonization and gut microbiome composition was evaluated using chicken as a model organism
In the chicken group fed with L C+mcra, colonies of Lactobacillus in fecal shedding gradually increased, whereas fecal shedding of Lactobacillus in the wild-type Lactobacillus casei (LC) fed group and control group were observed to be noticeably lower than that in the LC+mcra fed group (Fig. 1D)
We showed the antagonistic effect of conjugated linoleic acid over-producing probiotic, L C+mcra, on enteric bacterial pathogens through in vitro s tudies[28,30,31]
Summary
The effect of sustainable probiotics on Campylobacter jejuni colonization and gut microbiome composition was evaluated using chicken as a model organism. The prevalence pattern of C. jejuni in pasture poultry and their sensitivity to various antibiotics indicate that animal farming without antibiotic and/or synthetic chemical growth promoters diminishes the prevalence of multiple antibiotic resistance C. jejuni in poultry, without altering colonization of other bacteria[5,6] Another important zoonotic pathogen is Salmonella enterica, with a higher prevalence in organic farming systems than in conventional farming systems, but a lower incidence of multi-antibiotic resistance in their organic c ounterparts[7, 8]. According to Wang, it is reported that prebiotics must not be digested in the upper portion of the gastrointestinal tract before reaching large intestine, where they are selectively fermented by beneficial and/or commensal intestinal microbes[19] Such type of fermentation leads to the changes in metabolic processes of gut microbes and nutrients availability in gut ecosystem, possessing a beneficial effect on the host. Bacterial strains usually used for synbiotic include but not limited to Lactobacillus spp., Bifidobacteria spp. and several prebiotics mostly form natural food sources like cocoa, p eanut[21]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
