Abstract
The article describes a highly sensitive single-step microplate enzyme immunoassay of the ELISA type for fullerene C60 and its derivatives. Monoclonal anti-fullerene antibodies and a conjugate between fullerene and horseradish peroxidase were used as specific reagents. A direct competitive ELISA was carried out that was based on antibodies immobilized in the well of a microtiter plate, a peroxidase-labeled antigen, and detection via the dye formed from 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide. Both pristine fullerene C60 and its water-soluble forms can be determined. The detection limits are 1.5 ng∙mL−1 for fullerene C60, and between 0.1 and 1.3 ng∙mL−1 for its derivatives. This ELISA format allows for almost two-fold reduction of the time needed for the assay in comparison to indirect scheme with labeled antibodies.
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