Abstract
This review is concerned with those competitive radioimmunoassays that are utilized for determination of the concentration of human serum proteins. Radioimmunoassays that measure the levels of the immunoglbulins, complement component C3, plasminogen, fibrinogen, hemopexin, haptoglobin, thyroxine-binding globulin, retinol-binding protein, albumin, and histocompatibility antigens are discussed. In these radioimmunoassays, which employ indirect or competitive binding, the amount of unlabeled antigen is directly proportional to the degree of inhibition it exerts on the reaction between antibody and isotope-labeled antigen. To perform the radioassay, one needs an extensively purified antigen, high affinity antibodies, and an effective means of separating the free from the bound antigen. The assays have proven to be sensitive, efficient, and economical. They are invaluable in detecting minute quantities of proteins in serum and other body fluids and are useful in evaluating structural similarities between proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
