Abstract
AbstractThe binding of the anionic dye 2‐(4′‐hydroxyphenylazo) benzoic acid (HABA) to bovine serum albumin (BSA) at pH 6.0–7.5 was studied by spectrophotometry. The values of the dissociation constants were obtained by use of a modified Langmuirtype equation for HABA. Competitive binding of the polyanions sodium poly(styrene sulfonate) (PSSNa), potassium poly(vinyl sulfate) (PVSK), poly(acrylic acid) (PAA), and poly(methacrylic acid) (PMAA), with HABA to BSA were evaluated by variations in the difference spectra of BSA‐HABA‐polymer systems.
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