Abstract
Carbamylcholine stimulated [ 3H]inositol phosphate accumulation in mouse cerebral cortical slices with an ED 50 value of approximately 70 μM. Increasing concentrations of the M 2 selective muscarinic cholinergic receptor antagonist, AF-DX 116 (0.3–3.0 μM), produced parallel shifts to the right for concentration-response curves to carbamylcholine. A pA 2 value for AF-DX 116 of 6.5 (low affinity) was obtained frommSchild plot analysis. It is concluded that the M 2 muscarinic receptor subtype, as defined by high affinity [ 3H]AF-DX 116 radioligand binding, is not appreciably coupled to polyphosphoinositide hydrolysis in the mouse cerebral cortex.
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