Abstract

Abstract A novel method for immunoassay is reported. A competitive assay for dinitrophenylated bovine gamma globulin (DNP-GGB) was performed using anti-DNP serum and DNP-sheep red blood cells (DNP-SRBC). After complement-mediated hemolytic reaction, the chemiluminescent measurements were performed without any separation procedure. The sensitivity was 0.1 μg/ml of DNP-BGG.

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