Abstract
An indirect ELISA was developed to quantify aflatoxin B1 (AFB1). The detection limit was 0.025 ng mlâ1. The test used polyvinyl chloride (PVC) plates, activated with AFB1 bound to bovine serum albumin (BSA). Polyclonal antibodies were raised in rabbits against AFB1âBSA. The specific antiâAFB1antibodies were recovered from the crude antiserum by affinity chromatography from a column containing immobilized BSA on Nylon 6â6. Goat antiârabbit IgG antibodies bound to peroxidase were used to detect the rabbit IgG antiâAFB1 antibodies bound to PVC plates. The colour developed by the subsequent enzyme conversion of the substrate was detected by spectrophotometry. The developed colour gave clear absorbance differences at varying doses of AFB1. Crossâreactivity with aflatoxin B2, aflatoxin G1 and aflatoxin G2 was measured, showing percentages of 5.43, 64.5 and 5.07 respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have