Abstract

A model of multicomponent multilayer adsorption has been developed to describe the simultaneous adsorption of lysozyme (14.3 kDa, pI = 11.35) and myoglobin (17.6 kDa, pI = 7.2) on mesostructured cellular foam (MCF) silica in 0.02 M potassium phosphate buffer at pH 7 and 25 °C. The mechanism of adsorption was proposed based on batch adsorption isotherms and flow microcalorimetry (FMC) traces for the enthalpy of adsorption. Thermodynamic derivations of Guggenheim–Anderson–deBoer (GAB) and Langmuir models were used to develop the model based on the proposed mechanism. Regression analysis demonstrated the effect of each of the proposed mechanistic equations.

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