Abstract
The use of the GUS reporter gene was found to be very suitable for studying the competitiveness of the Rhizobium strain NGR234 and Bradyrhizobium strain CP283 for nodulation in siratro. However, the expression from the transposon mTn5SSgusA20 declined in the nodules of old plants, particularly the nodules at 35 d after inoculation and onward. Siratro inoculated with both gusA-marked NGR234 and CP283 strains showed a similar nodulation and acetylene reduction activity (ARA) to those of their parental strains. No major changes in nodulation and symbiotic properties in these marked strains were observed. When the Rhizobium strain NGR234 and Bradyrhizobium strain CP283 were inoculated separately to siratro, both of them were found equally effective for nodulation in the plant. But when the Rhizobium strain NGR234, and gusAmarked Bradyrhizobium strain CP283 were co-inoculated to the plants in a ratio of 1:1, the strain NGR234 occupied 75% of the nodules, while, the strain CP283 occupied only 25%, irrespective of the growth stages and inoculum concentrations. Similar results were also observed in the plants in which the Bradyrhizobium strain CP283 and gusA-marked Rhizobium strain NGR234 had been co-inoculated. Thus, the Rhizobium strain NGR234 was more competitive for nodulation in siratro.
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