Abstract
A novel method for minimizing differences in retention times between different gradient high-performance liquid chromatography (HPLC) systems is proposed. The gradient profile ( i.e., mobile phase strength vs. time) reaching the column is duplicated on each gradient system by adjusting an initial isocratic period in the assay to compensate for the measured delay volume of that system. an initial isocratic period must be included inthe gradient profile to facilitate this approach. Suggested initial isocratic periods are 5 ml for standard HPLC systems and 0.5 ml for micro-bore systems.
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