Abstract
Specific race interactions between Colletotrichum trifolii and cultivars of resistant and susceptible alfalfa were examined in nonwounded excised cotyledons. Cotyledons in incompatible and compatible interactions exhibited visible differential reactions within 7-10 days after inoculation. C. trifolii infected alfalfa by directly penetrating the epidermis via a penetration peg from the appressorium. Appressoria matured within 20 h on resistant and susceptible cotyledons, but primary hyphae did not develop on resistant cotyledons. Phenylalanine ammonia-lyase and the pterocarpan phytoalexin medicarpin began to increase in resistant tissues after 24 h. Cotyledon tissues apparently became resistant prior to significant increases in medicarpin [...]
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