Compatibility of Native and Imported Entomopathogenic Nematodes with Different Applications of Insecticides for Controlling the Cigarette Beetle, Lasioderma serricorne Fabricius (Coleoptera: Anobiidae)

Ramadan M El-Ashry ,M Hegab

doi:10.21608/eajbsa.2021.198979

Abstract

The cigarette beetle, Lasioderma serricorne Fabricius (Coleoptera: Anobiidae), is an important pest of stored products in the world which caused major economic losses that required many strategies for controlling insect pest. This study investigated native entomopathogenic nematodes (EPNs) and imported EPN species alone at different IJs concentrations or in combination with three application rates (recommended, quarter and half recommended concentrations) of lambda-cyhalothrin, emamectin benzoate and indoxacarb against the third and the fourth larval instars of its pest under laboratory conditions. The results showed that The native H.bacteriophora (Ar-4 strain) and the imported H.bacteriophora (HP88 strain) were more effective against the fourth larval instar of L. serricorne than the third instar followed by S.carpocapsae (All strain) while H.bacteriophora (Ba-1 strain) and S. feltiae strain were the least efficacy. According to LT50 of tested EPNs, 100 IJs/larva was the best concentration used against the larvae instars of L. serricorne. As well as, the fourth instar was more susceptible to the tested applications of pesticides than the third larval instar. Moreover, The application with a quarter recommended dose of indoxacarb was the most toxicity application against the fourth instar when compared to lambda-cyhalothrin andemamectin benzoate. On the other hand, the effects of the interactions between native and imported EPNs varied greatly as stated by application rates of tested insecticides and juveniles' concentration of EPNs. It is concluded that the best interaction effect was obtained with quarter recommended application (0.25 RC) of lambda-cyhalothrin when combined with EPNs at 100 IJs/larva, which displayed synergistic effect to overcome incompatibility particularly with heterorhabditid species, the effect that could be beneficial when making progress the integrated program for stored-product pests.

Highlights

Larval stages feeding of stored grains caused the greatest damage to commodities by widespread and destructive cigarette beetle, Lasioderma serricorne Fabricius (Coleoptera: Anobiidae) (Mahroof and Phillips, 2008)
1-Pathogenicity of Different Entomopathogenic Nematodes (EPNs) Concentrations Against the Third and The Fourth Larval Instars of Lasioderma serricorne Fabricius under Laboratory Conditions: a- Percentage of Mortality of The Third Larval Instar of L. serricorne : The third instar larvae of L. serricorne treated with 50,100,200 and 250 infective juveniles (IJs) concentrations of entomopathogenic nematodes (EPNs) species demonstrated a significant level of mortality percentages with native and imported nematode species (Tukey’s test: = 0.05).The least mortality percentage was observed at 50 IJs/larva (12.0 %) with native H.bacteriophora (Ba-1) and imported S. feltiae (Filipjev) whereas, the high mortality percentages (22.0 and 28.0 %) were found with native H.bacteriophora (Ar-4) and imported H.bacteriophora (HP88) species, respectively at five days post-application
Larvae mortality did not demonstrate significant differences between S.carpocapsae (All), H.bacteriophora (Ar-4) and H. indica at applied concentration 100IJs/larva with mortality percentages between 46 and 54 % as well as, the least mortality percent (16.0%) was achieved by H.bacteriophora (Ba-1) at five days post nematode application

Summary

Introduction

Larval stages feeding of stored grains caused the greatest damage to commodities by widespread and destructive cigarette beetle, Lasioderma serricorne Fabricius (Coleoptera: Anobiidae) (Mahroof and Phillips, 2008). 0.25 RC of Tested Chemical Pesticides: To evaluate the joint application of insecticides, emamectin benzoate, indoxacarb, and lambda-cyhalothrin and EPNs (100 IJs/larva) in controlling L.serricorne, ten active larval instars (3rd or 4th larval instars) were placed in 5-cm diameter Petri dishes lined with moist two filter paper.

Results

Conclusion