Abstract
With the rapid development of sports, sports injuries have become an inevitable problem in sports. Repairing sports injuries has always been the focus of attention in the field of sports and medicine. The wide application of biomedical materials leads to the innovation and development of medical technology. It is of great significance to use them to repair sports injuries, but the current research results are not ideal. In this paper, the biocompatibility of composite biomaterials used to repair sports injuries was studied. First, the rat bone marrow mesenchymal stem cells were cultured to prepare a cell suspension. Then, the tricalcium phosphate‐gelatin composite scaffold material and the collagen material were scraped into powder to make the material extract, which are, respectively, the experimental group and the control group. An apoptosis detection kit and a flow cytometer were used to detect apoptosis rate and survival rate of all samples, and a CKK‐8 kit was used to detect cytotoxicity and calculate cell proliferation activity. The absorbance value was read with a microplate reader, and the coagulation performance and hemolysis rate of different groups were calculated. The experimental results showed that the cell survival rate of the experimental group was 91.41%, the early apoptosis rate was 2.81%, the stem cell proliferation activity was 0.83, and the anticoagulant performance and hemolysis rate were 64.8% and 0.28%, respectively. This indicates that although the anticoagulant performance of the tricalcium phosphate‐gelatin composite scaffold is weak, its cell proliferation activity is strong, which is more suitable for cell adhesion and proliferation, and the cell apoptosis and hemolysis rate are lower. Therefore, it has better biocompatibility than a single collagen material.
Highlights
IntroductionWhen sports injury is serious, it is necessary to implant biomaterials to replace damaged tissues or organs for repair
Since the experimental group has the highest number of living cells and a lower apoptosis rate, it indicates that the experimental group has a higher cell survival rate and better biocompatibility
In the control group with high absorbance, the collagen material has better anticoagulant performance at 68.8%, while the tricalcium phosphate-gelatin composite scaffold material may have a certain cytotoxicity during the crosslinking process, which is related to the phosphoric acid on the cell membrane surface
Summary
When sports injury is serious, it is necessary to implant biomaterials to replace damaged tissues or organs for repair. For the composite biomaterials which combine the advantages of a variety of materials, it is necessary to study the biocompatibility of these new composite materials, so as to ensure that there will be no new damage to the human body after implantation. Bioremediation materials include the following categories: (1) biomedical metal materials: medical metal materials are metals or alloys used as biomedical materials, which have high mechanical strength and fatigue resistance, and are the most widely used clinically for load-bearing implants. Titanium alloy and polyethylene tissue prostheses are often used as joint materials; carbon-titanium synthetic materials are good artificial femoral heads for clinical applications; polymer materials and biopolymers (such as enzymes, antibodies, antibodies, and hormones) can be combined as a biosensor
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