Abstract
Abstract The distribution of glucose 6-phosphate dehydrogenase and D-glyceraldehyde 3-phosphate: NADP reductase activities in maize leaf blades was investigated by differential tissue fractionation. The reliability of this technique was verified on the basis of distribution of several marker enzymes (NADP-malate dehydrogenase and phosphoenolpyruvate carboxylase for mesophyll cells, NADP-malic enzyme and fructose 1,6-bisphosphate aldolase for bundle-sheath cells). Glucose 6-phosphate dehydrogenase is found mainly (70%) in the mesophyll, similar to the triose phosphate dehydrogenases, both NAD- and NAD(P)-dependent. D-glyceraldehyde 3-phosphate: NADP reductase occurs 75% in the mesophyll. Neither enzyme is found in chloroplasts. Specific activities of nine leaf enzymes were followed for up to 7 days after exposure of 4-day-old dark-grown seedlings to the light. While several activities related to C4 photosynthesis (aldolase, phosphoenolpyruvate carboxylase, NADP-glyceraldehyde 3-phosphate dehydrogenase, NAD...
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