Abstract
Intracellular compartmentation of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) and N‐malonyl‐1‐aminocyclopropane‐1‐carboxylic acid (MACC) in wheat (Triticum aestivum L. cv. Kanzler) and barley (Hordeum vulgare L. cv. Gerbel) leaves was studied using different methods: first, the isolation of intact vacuoles from protoplasts and, second, a non‐aqueous fractionation procedure. The two methods gave similar results. ACC concentrations were similar in the extravacuolar space and in the vacuole, whereas MACC was accumulated in the vacuolar space. Transport studies revealed that no specific carrier for ACC exists at the tonoplast. MACC transfer across the tonoplast was enhanced by 120% in the presence of ATP. MACC competitively inhibited malate transport into the vacuole indicating that the same transfer system catalyzes the transfer of the two dicarboxylates.It is concluded that malonylation of ACC is not a prerequisite for the transport of ACC through the tonoplast.
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