Abstract
The Rhizobium was isolated from the plant root and purified. The isolated rhizobium sample was identified based on morphological and bio chemical characterization. The result of biochemical tests showed positive result in indole test, citrate utilization test,starch hydrolysis test, urea hydrolysis test and catalase test. The isolated culture was mass multiplied and used by seed treatment like imbibed treatment and germinated treatment. After treatment the seedlings per planted separately by part culture method. The result showed that the rhizobium inoculation on the emerging radicals of one day old seedling is highly effective in nodulation and biomass production respectively
Highlights
Nitrogen is one of the major important nutrients very essential for crop growth
The present study was undertaken with the view to comparative study on the effect of Rhizobium inoculation on imbedded seeds and germinated seeds of Black gram (Vigno mungo L.)
In the present study rhizobium was isolated by root nodules of legume plant Vigno mungo .The isolates were purified and showed positive result in PHB test and failed to congo red in YEMA coloration was not found in lactose agar test, 3- keto lactose test and no growth was found in Hofer’s alkaline agar and litmus milk reaction test of rhizobial isolates
Summary
Nitrogen is one of the major important nutrients very essential for crop growth. But the availability of this element in soil is naturally deficient (Bras et al, 2000).so the nitrogen requirements of crop plants met by the addition of fertilizer nitrogen. Legume plants fix and utilize this nitrogen by working symbiotically with special bacteria called Rhizobium in nodules on their roots (Chandra,1991). While the potential for nitrogen fixation is directly related to the rhizobial survival, the extent of effective nodulation and the plant growth factors (Gandhi and Godbole, 1990).
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