Abstract
Abstract The use of the collagenase-SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) technique for comparing the condition of collagen in skin tissues is described. The technique was used on untreated skin samples and on others subjected to chemical treatments typically used for the preservation of biological specimens. Compounds examined included aluminum ammonium sulfate, arsenic trioxide, magnesium carbonate, mercuric chloride, potassium nitrate, sodium borate, sodium chloride, sodium fluorosilicate and formaldehyde. Differences were detected between the better preservatives, such as formaldehyde and arsenic trioxide, and others that actually promoted collagen degradation, such as potassium nitrate and mercuric chloride.
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