Abstract
The agreement between bioassay and immunoassay estimates of rat LH concentrations was examined in a series of interlaboratory comparisons of rat serum and pituitary extract. In each of 3 studies, aliquots of a pool of serum and of a pool of pituitary extract were assayed by both OAAD (ovarian ascorbic acid depletion) bioassay and by a radioimmunoassay (RIA) for rat LH. Each type of assay (OAAD or RIA) was performed in 3 laboratories. Among these laboratories, RIA procedures varied little but several variants of the OAAD assay were used, including the highly sensitive modification (“Est-Rx”) in which recipient rats are pretreated with estrogens. Estimates of the pituitary: serum LH ratio by “standard” OAAD assay were usually (but not invariably) lower than those obtained by the other 2 systems (“Est-Rx” bioassay and RIA, which agreed closely with each other). These lower estimates reflected relative overestimation of serum LH rather than underestimation of pituitary LH. This error seems attributable, in pa...
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