Abstract

This study was carried out to detect the Cryptosporidium spp in Karbala province, Iraq from. December 2019 to September 2020. Age, sex, and months interference with parasite prevalence were studied. A total of 100 fecal samples were collected from adults and young and from both sexes of human. Fecal samples were subjected to conventional methods (Flotation Methods by Sheather's sugar solution and stained with modified Ziehl-Neelsen) for parasite diagnosis. The result recorded that the infection rate of Cryptosporidium spp was 26%. The age group of 2-6 years had the highest infection rate comparing to other age groups ranged from 12-25 years yet was marginal significant (P<0.06). Regarding sex, there was no significant differences in infection rate, although the males recorded numerically higher rate of prevalence. The rate of infection of Cryptosporidium spp were varied among months, where in February recorded 46.66% in contrast to 10% recorded in July. It can be concluded that variables studied (age, sex, and months) have no influence on Cryptosporidium prevalence in Karbala province.

Highlights

  • Cryptosporidium, a protozoan parasite belongs to the Phylum Apicomplexa and Family Cryptosporidiidae, is a common cause of diarrhea in man, domestic animals, and wild vertebrates [1]

  • Fecal samples were subjected to conventional methods (Flotation Methods by Sheather's sugar solution and stained with modified Ziehl-Neelsen) for parasite diagnosis

  • This study aimed to investigate human patient infections with cryptosporidiosis by most two conventional methods Sheather's sugar solution and stained with modified Ziehl-Neelsen staining technique

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Summary

Introduction

Cryptosporidium, a protozoan parasite belongs to the Phylum Apicomplexa and Family Cryptosporidiidae, is a common cause of diarrhea in man, domestic animals, and wild vertebrates [1]. Infection with cryptosporidiosis in man was observed in 1976 [2]. The disease belonged to be one of the most serious infection that cause an intestinal infection of man, animals, and birds. Human and animal may take the infection by eating and drinking polluted water and food with oocysts of this parasite. Twenty-three species and sixty-one valid genotypes of Cryptosporidium spp. have been studied from a wide range including humans, mammals, birds, domestic livestock, wildlife, reptile, amphibians, and fish which can be causing asymptomatic or mild-to severe gastrointestinal disease in its host species [6]. This study aimed to investigate human patient infections with cryptosporidiosis by most two conventional methods Sheather's sugar solution and stained with modified Ziehl-Neelsen staining technique

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