Comparison on the effect of MART-10 and calcitriol on the treatmentof non-small cell lung cancer by determining which one is a morepotent drug in treating non-small cell lung cancer

Abstract

Non-small cell lung cancer is one of the most common types of lung cancer, with a high mortality rate worldwide.The current treatment methods have apparent side effects and inevitable drawbacks. A compelling new drug is badlyin need of development. A previous study has reported that calcitriol and MART-10 can effectively block anaplasticthyroid cancer (ATC) spread. MART -10 is more potent than calcitriol in inhibiting migration and invasion of ATC.This study investigates the effect of NSCLC using MART-10 and calcitriol and finds out which is more potent, both invitro and in vivo. The experiments will use known human non-small cell lung cancer cell lines (A549 cell line) and twovitamin D compounds: MART-10 and calcitriol and Xenograft mouse system. MTT measures cell proliferation. FACSmeasures cell apoptosis for AnnexinV and PI. Measure development in tumor size by mouse xengraft system. A positivecontrol is taxol, and a negative control is PBS. There are three most possible results: (1) MART-10 and calcitriol cankill A549 lung cancer cells, and MART-10 reduce the number of A549 lung cancer cells at a lower concentration thanwith calcitriol. (2) MART-10 and calcitriol can kill A549 lung cancer cells, and calcitriol reduces the number of A549lung cancer cells at a lower concentration than with MART-10. (3) MART-10 and calcitriol do not significantly killA549 lung cancer cells. The result of the study will provide critical information for the future clinical trial of MART10 and calcitriol in treating NSCLC. It would increase our understanding of the more effective treatment of NSCLC andimprove human health. Future studies should focus on combinations of MART-10 or calcitriol with various anti-cancerchemotherapy drugs.