Abstract
To study on the morphological character of cytology and physiological and biochemical mechanism of alfalfa cytoplasmic male sterile line, this paper took sterile line MS-GN1A and maintainer line MS-GN1B as raw material, separately conducted microscopic observation to anther in different development stage and determined the content of soluble sugar, starch, soluble protein, free proline and malondialdehyde, and the activity of antioxidant enzyme (catalase, peroxidase and superoxide dismutase). The results showed that: 1. Compared with maintainer line, the pollen grain of alfalfa cytoplasmic male sterile line presented shriveled appearance, smaller size with deletion and few; during the tetrad period of pollen, tapetum cell abnormally grew; callose and epidermis didn’t disintegrate and couldn’t release pollen grain; pollen abortion of alfalfa cytoplasmic sterile line was related to the abnormal development of connective vascular bundle. 2. With the growth and development of bud and the development and maturity of pollen, the content of soluble sugar, starch, soluble protein, and free proline showed deficient phenomenon in each development period of bud of sterile line MS-GN1A; as peroxidation index of membranous substance, the activity of malondialdehyde and antioxidant enzyme was higher than it of homotype maintainer line MS-GN1B.
Highlights
The acquisition of male sterile lines is of great significance for the utilization of plant heterosis
The change range of cytoplasmic male sterile lines from stage IV to stage V is relatively large, and the maximum change range of maintainer lines is from stage III to stage v. the highest value of soluble sugar content of CMS lines is more than the lowest value of soluble sugar content of maintainer lines.D
The vascular bundle of drug septum is a channel for plants to absorb water and transport nutrients to the anther chamber for pollen formation and development [13].The main reason is that the metabolism of the drug septum vascular bundle and the drug chamber is disordered during the normal development of microspore, which results in the loss of nutrients in the spore
Summary
The acquisition of male sterile lines is of great significance for the utilization of plant heterosis.
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