Comparison of Yersinia enterocolitica DNA Isolation from Swabs Without Pre-Enrichment on Selective Media With Isolation Preceded by Warm and Cold Enrichment.

Abstract

Bacteriological methods for the identification of Yersinia enterocolitica are laborious and time-consuming. The aim of this study was to compare Y. enterocolitica DNA isolation from swabs without pre-enrichment on selective media with isolation preceded by warm and cold enrichment. The material for the study consisted of 150 rectal swabs taken from 50 clinically healthy fattening pigs. Forty-one Y. enterocolitica strains were isolated after warm enrichment and 43 after cold enrichment. The presence of ail, ystA, and yadA gene fragments was detected in all isolates. DNA isolation from swabs without pre-enrichment supported the detection of Y. enterocolitica in only nine samples. This study demonstrates that Y. enterocolitica DNA can be isolated from swabs without pre-enrichment on selective media, but this method is less sensitive than the approach where DNA isolation is preceded by warm or cold enrichment.