Abstract
The lithium ions concentration in human serum was determined using Dry-slide technology Vitros 250 Analyser (Ortho Clinical Diagnostic), atomic absorption spectrometry (AAS) method Perkin Elmer 403 and ion-selective electrode (ISE) potentiometry AVL 9181. We compared lithium ions results in sample sera between these methods. Our reference method was AAS. We analyzed lithium ions concentration in 23 sera samples of patients after oral administration of lithium carbonate (3x 300mg) Jadran, Galen Laboratory Rijeka, by dry-slide technology, AAS and ISE methods. The quality control, precision, reproducibility and accuracy for Vitros dry slide technology were assessed. We established that the main difference between AAS method and dry slide technology was not statistically significant at p< 0.05 according to Student t-test. Therefore, the dry slide technology may be a useful alternative or it may even replace other methods, such as AAS. The main difference between dry slide technology and ISE methods was statistically significant at p<0.05 using Student t-test. By ISE method, we obtained considerably higher results, which may be explained by the presence of electrolytes or medicaments interfering with lithium ions.
Highlights
Lithium carbonate was introduced into psychiatry in 1949 in treatment of mania
We established that the main difference between atomic absorption spectrometry (AAS) method and dry slide technology was not statistically significant at p< 0.05 according to Student t-test
Atomic absorption spectrometry (AAS) and ion-selective elec trode (ISE) potentiometry are the techniques used in quantitative determination of serum lithium concentra tion
Summary
Lithium carbonate was introduced into psychiatry in 1949 in treatment of mania. In 1970, the US Food and Drug Administration approved the use of lithium com pounds in treatment of the manic phase of manic-de pressive disorder (bipolar disorder). It is considered that lithium reduces the frequency of manic episodes and, with their repetition, reduces episode intensity. It pre vents and stabilizes repetitive bipolar disorders without provoking sedation, amnesia and/or inhibition of mental processes. Therapeutic range for lithium in serum is narrow (0.6-1.2 mmol/L), and patients have to be monitored for levels of the drug constantly because of its potential toxicity. Vitros dry slide technology method consists of a colorimetric end-point reaction where the crown-ether chromophore binds lithium in the sample, the resultant dye complex is measured using reflectance spectropho tometry. Using patient samples collected in our laboratory we analyzed lithium concentrations by Vitros dry slide technol ogy, AAS and ISE methods and compared the results
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