Abstract
Vitrification is a freezing method that is applied in almost every IVF clinic in Japan. In our clinic, human embryos are vitrified by Cryotop method in which the solution containing embryos is directly exposed to liquid nitrogen. This method does not completely eliminate the risk of cross-contamination during their storage. Therefore, the closed vitrification device, Rapid-i, has been developed to solve this problem. In the present study, survival rate and subsequent development of warmed embryos were compared between Cryotop and Rapid-i, to evaluate if embryos can be safely cryopreserved by Rapid-i without sacrificing their potential after warming.
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