Abstract
TLC-DPPH• test coupled with different image processing procedures have become an interesting technique to screen complex samples for the presence of free radical scavengers. Several procedures have already been published showing the possibility of obtaining quantitative data from TLC plates derivatized with methanolic solution of relatively stable free radical DPPH• (2,2-diphenyl-1-picrylhydrazyl). In this study two different image processing programs, ImageJ and Sorbfil TLC Videodensitometer, have been applied to study direct antioxidant properties of compounds separated from extracts obtained from selected varieties of Medicago sativa. M. sativa var. Radius possessed the greatest amount of compounds with direct antioxidant properties. The activity of the individual compounds has been compared to the activity of rutin, a flavonoid with well recognized antioxidant properties. Neither of the separated compounds was as active as the standard.
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