Comparison of two methods to assess the tissue/blood partition coefficient for xenon in subcutaneous adipose tissue in man

Abstract

A new method to calculate the tissue/blood partition coefficient (lambda) for xenon in studies on the subcutaneous adipose tissue blood flow was compared with a previously reported method based on local skinfold thickness (lambda LST). The former method included needle biopsies from the abdominal and femoral subcutaneous adipose tissue, and the mean fat cell diameter was measured (lambda ECT). The extracellular tissue fraction in subcutaneous tissue was then estimated from a diagram. The tissue lipid content was approximated to equal the relative intracellular volume and Ostwald's solubility coefficients for 133Xe, based on the distribution of xenon in lipid, albumin and 0.9% saline were applied. Estimated lambda-values based on needle biopsies from the abdominal site were: 8.6 +/- 0.1 versus 9.9 +/- 0.4 ml g-1 (mean +/- SE) (P < 0.05) and from the femoral site: 9.1 +/- 0.1 versus 9.6 +/- 0.2 in lean (n = 10) and obese subjects (n = 10), respectively. The corresponding lambda-values obtained from skinfold measurements were: 6.2 +/- 0.5 versus 11.0 +/- 0.4 (P < 0.001) and 6.9 +/- 0.3 versus 11.4 +/- 0.4 (P < 0.001) in lean and obese subjects, respectively. Pooled lambda LST-values correlated positively with estimated adipose tissue blood flow (ATBF) (r: 0.34, P < 0.05, n = 40) whereas no such correlation was found for lambda ECT-values. In conclusion, a new method is presented which may allow an accurate determination of, and which may lead to reliable data on, subcutaneous ATBF in both lean and obese subjects.(ABSTRACT TRUNCATED AT 250 WORDS)