Abstract
Two methods, the colorimetric method (neutral red dye uptake), and DNA hybridization using a HSV thymidine kinase gene probe (TK) have been used to examine the sensitivity of 84 herpes simplex virus (HSV) type 1 and 2 clinical isolates to two antiviral drugs, acyclovir (ACV) and alpha-interferon (α-IFN). Using the colorimetric method, HSV isolates had ED 50s ranging from 0.03 ± 0.02 μg/ml to 0.164 ± 0.03 μg/ml for ACV and 6.3 ± 5.2 IU/ml to 55.0 ± 11.4 IU/ml for α-IFN. With the DNA hybridization method, ED 50s ranged from 0.033 ± 0.012 μg/ml to 0.190 ± 0.031 μg/ml for ACV and 8.5 ± 5.0 IU/ml to 43.5 ± 6.0 IU/ml for α-IFN. Two strains of HSV-1 were found to be resistant to very high concentrations of ACV (>50.0 μg/ml). The values obtained by the two methods showed good correlation ( r=0.724, P=0.002). Furthermore, our results demonstrate that the two methods are reproducible, reliable and the dye uptake assay is suitable for use in a diagnostic virology laboratory.
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