COMPARISON OF TWO METHODS IN PURIFICATION OF MEAT-DNA FOR PCR

Abstract

The Wizard Clean up System and a three-phase partitioning (TPP) method were used to purify genomic pork-DNA of various food samples for amplification. Quality of DNA purified by Wizard resin and partitioning was controlled by spectrophotometer and electrophoresis, respectively. A 108 bp fragment from the porcine growth hormone gene was applied, according to M EYER and co-workers (1994). Of all the samples prepared, amplicons were obtained by the pork- DNA specific PCR. Partitioning was found to be an efficient DNA purification step in preparation of PCR-grade DNA.