Abstract
BackgroundDirect methods for the assessment of intra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of intra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study. MethodsdIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion. ResultsMean dIEM and iIEM were 0.20 ± 0.04 mmol/1012 cells and 0.25 ± 0.04 mmol/1012 cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium. ConclusionsWe observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.
Highlights
Magnesium is the second most abundant intracellular cation and is essential for regulation of many functions within the cell, including modulation of ion channels, protein synthesis and energy metabolism [1]
We previously showed that lower plasma magnesium concentra tions were associated with increased risk of type 2 diabetes mellitus (T2DM) in women [4]
We found a positive correlation between plasma magnesium and dIEM (r = 0.18, P < 0.001) (Fig. 2A)
Summary
Magnesium is the second most abundant intracellular cation and is essential for regulation of many functions within the cell, including modulation of ion channels, protein synthesis and energy metabolism [1]. Direct methods for the assessment of intra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. Less labor intensive method is indirect calculation of intra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excre tion. Plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magne sium. Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable al ternative for the labor intensive dIEM method
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