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Abstract
Bones are important evidence often found in crime scene associated with mass grave disaster and missing persons. Substantial fragmentation and intermixing of bones limit the use of morphological observation. Human individualization using DNA analysis can complement but the challenges are the PCR inhibitors contained and low amount of DNA in degraded bone. The selection of effective DNA extraction method that minimizes PCR inhibitors and maximizes DNA recovery thus is crucial. This study aimed to compare the efficiency of the currently modified PCI-silica and the latest forensic commercial kit developed for challenging bone sample (PrepFiler® BTA). The results showed that PrepFiler® BTA provided significantly higher median DNA concentration (p < 0.05) and 95.3% probability of obtaining more alleles compared to modified PCI-silica based method. The efficiency of PCR inhibitors removal showed that both extraction methods were comparable at minimizing PCR inhibitors. Our study could serve as another guideline for selection of most suitable method for DNA analysis from bone.
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