Comparison of two different serological methods in the diagnosis of Rotavirus and Adenovirus in stool samples

Abstract

Objective: Rotaviruses are the most common cause of acute infectious diarrhea in children under 5 years of age worldwide. This study aims to compare the immunochromatographic and ELISA methods, determine the sensitivity and specificity of the methods used, and shed light on humanity to diagnose the disease correctly. Method: Stool samples of 1000 patients sent to Diyarbakır Pediatrics Hospital for rapid antigen testing of Rotavirus were studied. Single-stage Rotavirus and Adenovirus Co-immunochromatographic and ELISA methods were used in the examination of the samples. Samples with positive rapid test results were taken into 1.5 ml microcentrifuge tubes, and positive antigen samples collected for the ELISA test were stored at -80°C until the time to be studied. Results: Of 1000 stool samples analyzed, 40 (11.59%) of 345 girls and 60 (9.16%) of 655 boys had Rotavirus antigen in a total of 100 (10%) and 20 (2%) had Adenovirus antigen (P> 0.05). Viral antigen-positive cases were seen most frequently in winter months (December, January, February). There was no statistically significant difference between the data in terms of age and gender (P