Comparison of tuberculin skin test and lateral flow rapid test for detection of bovine tuberculosis in dairy cattle

Essam Nasr,Seham Gorge,Lilian Melika,M Marwah,Abeer Tammam

doi:10.21608/javs.2016.61824

Abstract

Tuberculosis is the most important zoonotic bacterial disease that is hazardous to both man and animals. A huge economic loss which could be direct or indirect is associated with the disease, so rapid diagnostic tests for tuberculosis are needed to facilitate early detection and prevention of disease transmission. The aim of this work is the detection of bovine tuberculosis by application of different serological tests. Tuberculin skin test applied on 1900 cattle, only 50 (2.6%) showed positive results, and then slaughtered. Forty five (90%) of slaughtered animals showed visible lesions on post mortem examination, while the other five (10%) showed non visible lesions. The bacteriological examination of the 50 samples reveled Mycobacterium bovis form 40 processed samples (80%). Results of Anigen Rapid Bovine TB Ab test and ELISA test had detected 42% and 48% of tuberculin positive cattle respectively. It was concluded that the Anigen Rapid Bovine TB Ab kit test is rapid, safe, simple and easy to perform and provide yes or no results within 15 to 20 minutes but it is not efficient for detection of bovine tuberculosis in cattle and could be useful as a complementary for tuberculin test.

Highlights

Bovine tuberculosis is a worldwide disease that causes a great harm on dairy farms and poses health risks to the population that consumes products of animal origin
The primary methods used for the detection of TB in humans and ruminants include the measurement of a delayed type hypersensitivity to purified protein derivative (PPD) and an indirect in vitro assay that measures the concentration of gamma interferon (IFN-γ) produced in response to stimulation with PPD (Monaghan et al, 1994, Wood et al, 1992, Wood and Jones 2001)
Results of Anigen Rapid Bovine TB Ab test kit from tuberculin reactor cattle in comparison to the type of lesions. It is cleared from table 3, that 21(42%) of tuberculin reactor cattle were positive with Ani- gen Rapid Bovine TB kit

Summary

Introduction

Bovine tuberculosis is a worldwide disease that causes a great harm on dairy farms and poses health risks to the population that consumes products of animal origin. A number of Enzyme Linked Immunosorbent Assays (ELISA) have been described based on complex M. bovis antigens, such as Purified Protein Derivatives (PPD) and phosphatide antigens. All of these assays were successful in detecting circulating antibodies to mycobacteria but have been considered to lack specificity (Mcnair et al, 2001)

Objectives

Methods

Results