Abstract
BackgroundMost of the locally available medicinal plant is used as medicine through different ways since the ancient time. Hyoscyamus gallagheri (H. gallagheri) is one of the rare medicinal plant which is indigenous to Oman as well as Gulf countries. The main target of this study is to estimate the total phenols and flavonoids content and evaluate the antioxidant activities of the aerial parts extracts which were collected from the southern part of Oman.MethodsInitially, the powder of aerial parts of the selected plant was extracted with a polar solvent. The prepare extract was the successive fractionation by different polarities of solvents. All the extracts were used for the estimation of total phenols and flavonoids content by common Folin–Ciocalteu reagent (FCR) and calorimetric methods. In addition, the extracts were evaluated for their antioxidant activity by spectroscopic technique through α,α-diphenyl-picrylhadrazyl (DPPH) method.ResultsIn the present experiment, the ethyl acetate extract contains the highest amount of phenols and the lowest in hexane extract. However, the highest amount of flavonoids was obtained from butanol extracts and the lowest in water aerial extract. In addition, the highest antioxidant activity showed in the ethyl acetate aerial extract and the lowest was in the hexane aerial extract. The other aerial extracts also showed the similar antioxidant activity comparable to Gallic acid standard.ConclusionIt was also observed that there is a significant correlation between the antioxidant and total phenols. Therefore, the best activity aerial extract might be used for the treatment of human diseases in near future.
Highlights
The search of relief from illness, through application of medicinally active plant crude drugs, has its origins in the distant pre-historic past
The solvents used for extraction were methanol, hexane, butanol, chloroform, dimethyl sulphoxide (DMSO), NaNO3, NaOH, and other general chemical and solvents were bought from Sigma-Aldrich Company Limited
Plant sample The aerial parts of H. gallagheri were collected in the month of September, 2018 from Dhofar and the collected plant was authenticated by local area people and Associate Professor Dr Syed Abdullah Gilani at the Department of Biological Sciences, University of Nizwa, Nizwa, Sultanate of Oman
Summary
The search of relief from illness, through application of medicinally active plant crude drugs, has its origins in the distant pre-historic past. Plant medicines are significant source of pharmaceuticals through several ways, which includes i) pure active compounds in their native form that are difficult or expensive compare to synthesize one, ii) as active compounds which have been modified to reduce toxicity and improve efficacy, iii) as models for development of synthetic analogs with similar biological activities, and iv) as inactive or poorly-active, but novel, chemical skeletons which promise interesting discoveries through modification [2, 3]. It is to contribute to pharmaceutical development, studies of the biological activity and chemistry of traditionally-used medicinal crude drugs should contribute to the rationalized use (2019) 5:20 of these plants by the human population that depends on them for daily, front-line health care [4, 5].
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