Abstract
The efficacy of three media (antibiotic-supplemented plate count agar (3 days, 21 °C); antibiotic–pyruvate–supplemented plate count agar (3 days, 21 °C); and trypticase soy agar (16 h, followed by 2.5 days on antibiotic-supplemented plate count agar, 21 °C)) for recovery of heat-injured Botrytis cinerea spores was compared using hydrophobic grid membrane filters. The filters restrict spreading of fungal colonies and facilitate the transfer of fungal spores from pre-enrichment trypticase soy agar to antibiotic-supplemented plate count agar. Counts obtained from pre-enrichment on trypticase soy agar were significantly larger than counts obtained from the other media (α = 0.05). Addition of sodium pyruvate to antibiotic-supplemented plate count agar did not increase spore recovery (α = 0.05). The use of trypticase soy agar as a pre-enrichment medium should be considered for use when antibiotic-supplemented plate count agar is employed in the enumeration of heat-stressed fungi.
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