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Abstract
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphate to human placental lactogen were investigated, involving water-soluble carbodiimide, glutaraldehyde, and the periodate oxidation technique. Of the 3, the periodate oxidation technique proved superior giving a conjugate with only slight changes in the Michaelis constant ( K m) and maximum velocity ( V max) which could be used for an enzyme immunoassay for human placental lactogen in human plasma.
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