Comparison of Three Chromatographic Systems for Determination of Organic Acids in Wine

Abstract

Three chromatographic systems: ion exchange chromatography, ion exclusion chromatography and reversed phase chromatography, have been used for the simultaneous determination of organic acids in wine. The common organic acids were separated using all three chromatographic systems. When an ion exchange column (TSKgel 1C Anion-PW) was used, organic acids and inorganic anions (CI- and SO42-) in wine were determined simultaneously without interference. The sharp peaks were obtained when an ion exclusion column (TSKgel OApak-A) was employed. A rapid separation of organic acids has been achieved, within about 7 min, when a reversed phase column (Zorbax ODS) was used. In ion exclusion and reversed phase systems combined with UV detection, however, other organic compounds which have ultraviolet absorption at 210 nm interfered with the determination of organic acids when practical wine samples were analyzed. IC is an obvious alternative for determination of organic acids in wine.