Abstract
Metal nanoparticles synthesized by green methods with the use of microorganisms are currently one of the most closely studied types of nanomaterials. It has accurately been shown that the characteristics of metal nanoparticles generated in the presence of different bacteria vary. For the two isogenic strains of obligate methylotrophic bacteria of the wild type (M. quaylei MTT) and its streptomycin-resistant mutant (M. quaylei SMR), the pleiotropic character of streptomycin resistance mutation in the SMR cells has been revealed. It has been shown that both cultures can generate silver nanoparticles. There is a dramatic difference in the formation of palladium nanoparticles, which are formed only in the presence of cells of the streptomycin-resistant mutant M. quaylei SMR. This study shows that closely related isogenic strains of obligate methylotrophic bacteria can be distinguished by the spectra of biogenic nanoparticles of two noble metals. While palladium nanoparticles are only generated by the cells of the streptomycin-resistant mutant M. quaylei SMR, biogenic silver nanoparticles can be generated from both cultures. Thus, the assessment of the ability of microorganisms to form biogenic nanoparticles of different metals allows the revelation of subtle metabolic differences of even close cultures.
Highlights
Methylotrophic bacteria are well known as objects of various biochemical and biotechnological studies [1,2,3,4] but not as producers of biogenic metal nanoparticles
The methanol dehydrogenase of methylotrophs catalyzes the first reaction in an unusual periplasmic electron transport chain responsible for the oxidation of methanol to formaldehyde, utilizing the Comparison of the Wild-Type Obligate Methylotrophic Bacterium Methylophilus quaylei and its Isogenic
The obligate methylotrophic bacteria deposited in the allRussian collection of microorganisms (VKM), M. quaylei (VKM B-2338) of the wild type [24] and its isogenic streptomycin-resistant SMR mutant, were used
Summary
Methylotrophic bacteria are well known as objects of various biochemical and biotechnological studies [1,2,3,4] but not as producers of biogenic metal nanoparticles. Gram-negative aerobic methylotrophic bacteria have prospects for use in nanobiotechnology for the biogenic synthesis of metal nanoparticles. Perhaps that is why methylotrophic microorganisms have been considered as industrial, biotechnological objects in the past few decades [5,6,7,8]. The high growth rate, the low price of growth media with methanol, and the plasticity of the regulation of growth parameters make it possible to consider methylotrophs as promising universal producers. Methylotrophic cells can use soluble periplasmic methanol dehydrogenase, which is an enzyme containing the prosthetic pyrroloquinoline quinone (PQQ) group. The methanol dehydrogenase of methylotrophs catalyzes the first reaction in an unusual periplasmic electron transport chain responsible for the oxidation of methanol to formaldehyde, utilizing the Comparison of the Wild-Type Obligate Methylotrophic Bacterium Methylophilus quaylei and its Isogenic
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