Comparison of the value of electrochemical luminescence immunoassay and enzyme-linked immunosorbent assay in the detection of human immunodeficiency virus

Abstract

Objective To investigate the value of electrochemical luminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA) in the detection of human immunodeficiency virus(HIV). Methods From March 2017 to March 2019, 12 680 samples of blood samples received by the laboratory of our hospital were included. The blood samples were initially screened by ECLIA method and ELISA method, and the positive samples were confirmed by Western blot method. To observe and compare the positive rate of initial screening of the two methods and evaluate the diagnostic value of the two methods. Results A total of 32 positive samples were detected by ELISA, and the positive rate was 0.252%. The positive rate of 30 samples detected by ECLIA method was 0.237%. There was no statistical difference in the positive rate between the two groups (P>0.05). The sensitivity, specificity, positive predictive value and negative predictive value of ELISA were 100%, 99.97%, 87.5% and 100%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value of ECLIA method were 100%, 99.98%, 93.33% and 100%, respectively. The area under the curve was 0.979 by ELISA and 0.981 by ECLIA. There was no statistical difference between the two groups (P>0.05). Conclusions The ECLIA method has high sensitivity and specificity, which is equivalent to ELISA method and has high application value. Key words: Electrochemical chemiluminescence immunoassay; Enzyme-linked immunosorbent assay; Human immunodeficiency virus; Diagnostic value