Abstract
BackgroundAflatoxin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Previous studies reported that these mycotoxins impaired mammalian oocyte quality. However, the effective concentrations of mycotoxins to animal oocytes were different.MethodsIn this study we aimed to compare the sensitivity of mouse and porcine oocytes to AFB1, DON, HT-2, OTA, and ZEA for mycotoxin research. We adopted the polar body extrusion rate of mouse and porcine oocyte as the standard for the effects of mycotoxins on oocyte maturation.Results and DiscussionOur results showed that 10 μM AFB1 and 1 μM DON significantly affected porcine oocyte maturation compared with 50 μM AFB1 and 2 μM DON on mouse oocytes. However, 10 nM HT-2 significantly affected mouse oocyte maturation compared with 50 nM HT-2 on porcine oocytes. Moreover, 5 μM OTA and 10 μM ZEA significantly affected porcine oocyte maturation compared with 300 μM OTA and 50 μM ZEA on mouse oocytes. In summary, our results showed that porcine oocytes were more sensitive to AFB1, DON, OTA, and ZEA than mouse oocytes except HT-2 toxin.
Highlights
Mycotoxins are secondary metabolites produced by fungi, while the most agriculturally common mycotoxins known today include aflatoxins (AF), deoxynivalenol (DON), T-2, ochratoxin A (OTA), zearalenone (ZEA) (Grajewski et al, 2012)
Effects of Aflatoxin B1 (AFB1) on mouse and porcine oocyte maturation We first examined the effects of AFB1 on mouse and porcine oocytes
Our results showed that with the same concentration of HT-2 treatment the rate of mouse oocyte polar body extrusion was lower than porcine oocytes, indicating that compared with porcine oocytes mouse oocytes were more sensitive to HT-2
Summary
Mycotoxins are secondary metabolites produced by fungi, while the most agriculturally common mycotoxins known today include aflatoxins (AF), deoxynivalenol (DON), T-2, ochratoxin A (OTA), zearalenone (ZEA) (Grajewski et al, 2012). These mycotoxins have multiple toxic effects on human and animal health in a very low dose, which draws worldwide attention. Aflatoxin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Our results showed that porcine oocytes were more sensitive to AFB1, DON, OTA, and ZEA than mouse oocytes except HT-2 toxin
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
