Comparison of the strychnidine and hydrazine methods for the estimation of nitrate in seawater : Dal Pont, G., B. Newell and J. Staniforth, 1963. Aust. J. Mar. Fresh. Res., 14 (1): 37–43

Abstract

Monoclonal anti-A, anti-B, anti-H antibodies have been produced by a murine hybridoma. C57BL/6 mice have been immunized by different red blood cells. The main characteristics of these antibodies are the following: - specificity: anti-A agglutinates only A cells, including A2, A3, Ax (from a panel of 50 cells); anti-B agglutinates only B cells (B3, Bx, cis AB and acquired B). Anti-H agglutinates all cells except H-deficient red cells. - inhibition: it is only inhibited by the saliva of each corresponding secretor subjects. - immunofluorescence tests: it is able to detect A, B, H antigens on tissue sections: kidney, pancreas, liver. - thermodynamics: the association constant is 2.107 1/ml (for anti-A) and 3.108 for anti-B and anti-H. All are IgM. Monoclonal anti-N, and some anti-public antibodies such as anti-gerbich and antiRh 17 have been obtained too. All these antibodies could be useful as reagents and for the study of weak groups.