Abstract
The aim of this study was to implement the determination of cardiac markers in preclinical research at our department. For this purpose, the pathophysiological model of acute cardiotoxicity induced by high doses of isoproterenol was used. Isoproterenol hydrochloride was intraperitoneally administrated to 42 Wistar male rats at a dose of 50 mg/kg body weight. Cardiac injury was determined by assessing the concentrations of the cardiac markers (cTnI - cardiospecific troponin I and CKMB - cardiac isoenzyme creatine kinase) in the blood at predetermined time-intervals (2, 4, 6, 12, 24 and 36 h), and confirmed by ECG. Isoproterenol hydrochloride caused an elevation in the plasma concentrations of both markers. The results showed a significant difference (P< 0.01) in the concentrations of cTnI between the experimental and control groups at 2, 4, 6 and 24 h with a maximum peak between the fourth and sixth hour. However, the difference in the concentrations of CKMB between the experimental and control groups was non-significant. This experiment confirmed that cTnI is more cardiospecific than CKMB. It also revealed the possibility to use this marker in preclinical testing.
Highlights
The aim of this study was to implement the determination of cardiac markers in preclinical research at our department
Cardiac injury was determined by assessing the concentrations of the cardiac markers in the blood at predetermined time-intervals (2, 4, 6, 12, 24 and 36 h), and confirmed by ECG
The results showed a significant difference (P < 0.01) in the concentrations of cTnI between the experimental and control groups at 2, 4, 6 and 24 h with a maximum peak between the fourth and sixth hour
Summary
The aim of this study was to implement the determination of cardiac markers in preclinical research at our department For this purpose, the pathophysiological model of acute cardiotoxicity induced by high doses of isoproterenol was used. Troponin (Tn) is a regulatory protein that forms a complex located in the contractile apparatus of the muscle tissue It consists of three subunits (C, T, and I) that differ in the role they play in the interaction between actin and myosin filaments (Adamcova et al 1999; Parmacek et al 2004). The highly preserved structure and function of troponins across animal species offer the option to employ the determination of troponins in preclinical research using highly sensitive human immunoassay kits (O’Brien et al 1997; Wells et al 2008) Their greatest potentials are seen in the development of myocardial protective strategies or predicting the cardiotoxicity of new chemical substances in animal models
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