Abstract
The use of various types of cyclodextrins (β-cyclodextrin, dimethyl-β-cyclodextrin, hydroxyethyl-β-cyclodextrin) as mobile phase additives were successful in the reversed-phase high-performance liquid chromatographic and micellar elektrokinetic chromatographic (MEKC) separation of fat-soluble vitamins. The use of MEKC over HPLC gave either comparable or improved resolution of these hydrophobic solutes. Both techniques proved successful in the separation of vitamins D 2 and D 3. Dimethyl-β-cyclodextrin as mobile phase and buffer additive provided the best separation of these two compounds. Generally, separation increased in both techniques with increasing cyclodextrin concentration. The addition of various cyclodextrins to the mobile phase aided in the separation of vitamins E and E acetate by HPLC with a phenyl stationary phase. The addition of cyclodextrin to the running buffer in MEKC gave little improvement in the resolution of these two compounds. In the separation of α-, β-, γ-, δ-tocopherol, the use of various derivatized β-cyclodextrins as HPLC mobile phase additives was not able to separate β- and γ-tocopherol; however the use of dimethyl-β-cyclodextrin as a buffer additive in MEKC resolved all four isomers.
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