Abstract
The forensic investigation of sexual crimes includes the use of presumptive tests, which are based on the detection of semen components like the enzyme acid phosphatase (AP) and the prostate specific antigen (PSA). Because the forensic field usually deals with trace and old samples, the major goal of this study is to evaluate the sensitivity and specificity of semen presumptive tests which have a specific forensic purpose, such as the colorimetric test kit SERI AP Spot Test and the immunochromatographic test kit SERATEC PSA Semiquant. To achieve this goal, samples containing saliva, semen, vaginal fluid, and mixtures were tested. The results of the AP Spot test indicate that the two minutes that limit the reaction time to avoid false-positive can be insufficient for detection of trace and old samples. The results of the PSA Semiquant suggest that this test is more sensitive and specific to semen detection than the AP test. Therefore, this study reassures the importance of presumptive tests as screening tests.
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