Abstract
Treatment of 30 different vegetables by the Pulsifier (30 s) and the Stomacher (60 s) showed that total viable cell counts and coliform counts were almost identical with less than 0.2 Log CFU/g difference and a ratio of ca. 1.00 between the two methods. However, the diluents from Pulsified samples were much clearer (Optical Density of 0.052), with less total soluble solids (0.30%) and slightly higher pH (6.56) than Stomached samples, which had more turbid liquid (Optical density of 0.237), with more total solids (0.55%) and lower pH (6.35). These data indicated that the Pulsifier provided equivalent viable cell count and coliform counts compared with the Stomacher from the 30 vegetables studied, but at the same time provided clearer liquid with less total soluble solid. This is advantageous for further microbiological manipulations such as Polymerase Chain Reaction, ATP evaluation, and immunological testing.
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