Comparison of the protocols for obtaining platelet-rich plasma in dogs: a cellular study

André William Masseaux Vidal Júnior,Heloísa Justen Moreira De Souza,Ana Paula Lopes Marques,Ricardo Siqueira Da Silva

doi:10.1590/0103-8478cr20180843

André William Masseaux Vidal Júnior, Heloísa Justen Moreira De Souza + Show 2 more

Open Access

https://doi.org/10.1590/0103-8478cr20180843

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Abstract

ABSTRACT: This study aimed to evaluate two protocols (PA and PB) that are used to obtain canine platelet-rich plasma (PRP) for cellularity. Twenty healthy dogs were used. Blood samples were collected and placed in two tubes of 3.2% sodium citrate. PA used double centrifugation at 210 x g, and 370 x g and PB used double centrifugation with 140 x g and 330 x g. The PRP samples from the protocols were examined in terms of their platelet, erythrocyte, and leukocyte count in the Neubauer chamber, differential leukocyte count and platelet morphological observation in blood smears. Data (mean and standard deviation) were analyzed with the 95% probability t-test (P <0.05) using Pearson’s correlation to test the relationship between platelets and erythrocytes, platelets, and leukocytes, and the leukocyte count versus the erythrocytes. Very weak negative correlation between platelets and leukocytes (p= -0.03), weak negative correlation between platelets and erythrocytes (p= -0.3) and a strong positive correlation between leukocytes and erythrocytes (ρ = 0.75) were noted. Although, BP did not reach the desired mean of one million platelets (979300 ± 79631 cells / μL), both protocols, A and B (4.42 ± 1.61 and 3.85 ± 1.55 times more platelets than total blood, respectively) (p <0.05) were efficient in concentrating platelets. Platelet activation was present in 26.55 ± 6.72% of the PA platelets and 26.25 ± 7.03% in PB (p> 0.05). PA and PB presented low erythrocyte concentration (p> 0.05), and PA had more leukocytes (p <0.05) than PB, with higher concentrations of basophils that were segmented, and lymphocytes.

Highlights

Platelet-rich plasma (PRP) is generally autologous and consists of a small volume of plasma with a high concentration of platelets, growth factors (GF), and cytokines for epithelial migration, bone and connective tissue formation (MARX, 2004; CROVETTI et al, 2004; HENDERSON et al, 2003)
Dogs can benefit from PRP, associated or not with other biomaterials, in bone grafts and bone tissue regeneration, and integration in cases of experimental fractures (YOU et al, 2007; CASATI et al, 2007)
The semi-automatic PRP processing method using a conventional centrifuge in a laboratory or outpatient environment provides low-cost PRP and greater applicability in veterinary medicine (VENDRAMIN et al, 2006)

Summary

Introduction

Platelet-rich plasma (PRP) is generally autologous and consists of a small volume of plasma with a high concentration of platelets, growth factors (GF), and cytokines for epithelial migration, bone and connective tissue formation (MARX, 2004; CROVETTI et al, 2004; HENDERSON et al, 2003). The PRP quality is mainly measured by the ability to concentrate platelets. The PRP should have three to five times more platelets than whole blood (WHITLOW et al, 2008). Other studies have indicated that a concentration > 1 million platelets/ μL is necessary (MARX et al, 1998; LEMOS, 2002). V.50, n.3, Dogs can benefit from PRP, associated or not with other biomaterials, in bone grafts and bone tissue regeneration, and integration in cases of experimental fractures (YOU et al, 2007; CASATI et al, 2007). The semi-automatic PRP processing method using a conventional centrifuge in a laboratory or outpatient environment provides low-cost PRP and greater applicability in veterinary medicine (VENDRAMIN et al, 2006)

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