Abstract
BackgroundRabies is an important viral zoonosis that causes acute encephalitis and death in mammals. To date, several recombinant vaccines have been developed based on G protein, which is considered to be the main antigen, and these vaccines are used for rabies control in many countries. Most recombinant viruses expressing RABV G protein retain the G gene from attenuated RABV. Not enough is currently known about the protective effect against RABV of a combination of recombinant adenoviruses expressing the G and N proteins of pathogenic street RABV.MethodsWe constructed a recombinant adenovirus (Ad-0910Gsped) expressing the signal peptide and ectodomain (sped) of G protein of the Korean street strain, and evaluated the immunological protection conferred by a single and combination of three kinds of recombinant adenoviruses (Ad-0910Gsped and Ad-0910G with or without Ad-0910 N) in mice.ResultsA combination of Ad-0910G and Ad-0910 N conferred improved immunity against intracranial challenge compared to single administration of Ad-0910G. The Ad-0910G virus, expressing the complete G protein, was more immunogenic than Ad-0910Gsped, which expressed a truncated G protein with the transmembrane and cytoplasmic domains removed. Additionally, oral vaccination using a combination of viruses led to complete protection.ConclusionsOur results suggest that this combination of viruses is a viable new intramuscular and oral vaccine candidate.
Highlights
Rabies is an important viral zoonosis that causes acute encephalitis and death in mammals
The amino acids at positions 459 to 524 of G protein, transmembrane and cytoplasmic domains were excluded from the construct of recombinant adenovirus Ad-0910Gsped for expression of sped (Fig. 1a)
Icosahedral capsids typical of adenovirus were observed in 293A cells infected with each recombinant adenovirus using electron microscopy (Data not shown)
Summary
Rabies is an important viral zoonosis that causes acute encephalitis and death in mammals. Rabies virus (RABV) is an important causative agent of viral zoonosis resulting in acute encephalitis and death in mammals. RNA, as well as a virion lipid envelope containing matrix protein (M) and glycoprotein (G) surrounding the RNP [1, 2]. Among these proteins, the G and N proteins are known to be important for immunogenicity against. The G protein is the major antigen in the formation of neutralizing antibodies (Nab) against RABV in animals [3,4,5]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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