Abstract
Human and rat peritoneal macrophages and rat Kupffer cells were labelled with [l− 14C] arachidonic acid and stimulated with the calcium ionophore A23187. The metabolites formed were separated by high pressure liquid chromatography (HPLC). Human peritoneal macrophages formed especially leukotriene B 4, 5-hydroxy-6,8,11,14 eicosatetraenoi acid and small amounts of leukotriene C 4 and thromboxane B 2, 12-hydroxy-5,8,10 heptadecatrienoic acid and 6-keto-prostaglandin F 1α, whereas rat peritoneal macrophages mainly produced cyclooxygenase products and in particular thromboxane B 2 and 12-hydroxy-5,8,10 heptadecatrienotic acid. Rat Kupffer cells synthesized mainly cyclooxygenase products such as prostaglandin F 2α, prostaglandin D 2 and prostaglandin E 2. These results indicate that the profile of eicosanoids production by macrophages is dependent both on the species and on the tissue from which the macrophage is derived.
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