Abstract
Abstract. Twenty healthy children were vaccinated, and then in a 5 years they were revaccinated with Priorix (measles, mumps, rubella vaccine), according to the national immunization calendar. The aim of the study was to observe and compare the shaping and maintenance of immunological memory. Specific IgM, IgA, IgG antibodies in serum, and their subclasses as well as antibodies avidity were tested by ELISA. It was demonstrated that IgM, IgG, and IgA antibodies shaped primary immune response to Priorix. Booster-effect was found in IgG and IgA antibodies in response to revaccination. Low avidity of antibodies in primary immune response changed to the high avidity (60–70%) antibodies of memory response to measles and rubella, and remained low for antimumps antibodies. IgG3 subclass was predominant, and IgG1 and IgG4 subclasses were involved in primary immune response. At the stage of immunological memory IgG1 was predominanted and IgG3 and IgG4 became minor.
Highlights
Twenty healthy children were vaccinated, and in a 5 years they were revaccinated with Priorix, according to the national immunization calendar
Specific IgM, IgA, IgG antibodies in serum, and their subclasses as well as antibodies avidity were tested by ELISA
It was demonstrated that IgM, IgG, and IgA antibodies shaped primary immune response to Priorix
Summary
СОПОСТАВЛЕНИЕ ПЕРВИЧНОГО И ВТОРИЧНОГО ГУМОРАЛЬНОГО ИММУННОГО ОТВЕТА НА ВАКЦИНАЦИЮ «ПРИОРИКС». С целью проследить и сопоставить особенности формирования, поддержания и функционирования иммунологической памяти, 20 здоровых детей, согласно российскому календарю прививок, были привиты вакциной Приорикс (корь, краснуха, паротит), а через 5 лет ревакцинированы той же вакциной. В сыворотке крови методом ИФА определяли специфические IgM, IgA и IgG антитела к кори, краснухе и паротиту, их авидность, а также субклассы специфических IgG. Антитела классов G и A сохраняются и на этапе иммунологической памяти, обнаруживая бустер-эффект при ревакцинации, особенно у лиц с низким уровнем антител. При ревакцинации авидность антител к кори, краснухе и эпидемическому паротиту не меняется. На этапе поддержания иммунологической памяти и ответа на ревакцинацию превалирует IgG1, а IgG3- и IgG4-антитела становятся минорными. Целью настоящей работы было проследить и сопоставить на одной и той же группе обследованных лиц особенности формирования, поддержания и функционирования иммунологической памяти на примере вакцинации и ревацинации Приорикс
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