Comparison of the patterns of milk serum proteins in subclinical mastitis caused by four common pathogens in dairy cows

Abstract

At the present time, one of the diagnostic methods supplemented with somatic cell count and bacterial culture is measuring the acute phase proteins in milk samples from cows with subclinical mastitis. Acute phase proteins are group of proteins that their increase (positive acute phase proteins) or decrease (negative acute phase proteins) can be a sign of inflammation, trauma, or infections in the mammary glands (Murata et al., 2004). Those acute phase proteins in serum and milk which are separable by cellulose acetate electrophoresis include albumin, immunoglobulin, α-lactalbomin and β-lactoglobulin. Although there is a good body of knowledge on serum milk proteins but the comparison of the effects of different pathogens causing subclinical mastitis on the patterns and changes of APPs in milk serum has not been studied yet. The objective of this study was to compare the increase or decrease patterns of serum milk APPs during subclinical mastitis caused by Streptococcus agalactiae, Staphylococcus aureus, Streptococcus uberis and Corynebacterium bovis and also to determine the correlation coefficients of these proteins with somatic cell counts as the gold standard of subclinical mastitis.KeywordsAcetate CelluloseMilk SampleAcute Phase ProteinSomatic Cell CountElectrophoretic PatternThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.